Flow cytometry is now a recognized methodology within animal spermatology, and has moved from being a research tool to become routine in the assessment of animal semen destined to breeding. The present review summarizes the state of the art regarding flow cytometry applied to animal andrology, albeit keeping an open comparative intent. It critically evaluates the present and future capabilities of flow cytometry for the diagnostics of potential fertility and for the development of current reproductive technologies such as sperm freezing, sperm selection and sperm sorting. The flow cytometry methods will probably further revolutionize our understanding of the sperm physiology and their functionality, and will undoubtedly extend its application in isolating many uncharacterized features of spermatozoa. However, continuous follow-up of the methods is a necessity owing to technical developments and the complexity of mapping spermatozoa. Evaluation of the spermatozoa of animals has either a purpose of diagnosis of the health of the male reproductive organs or, for those sires destined to breeding, of the odds their semen has to fertilize or, to lead to fertilization failure, depending on the approach to the problem.
WO2017173391A1 - Method to prepare sperm - Google Patents
WOA1 - Method to prepare sperm - Google Patents
Fernandez, Sendurai Mani , P. Rangarajan, P. The possibility of sperm as a vehicle to deliver foreign DNA to oocytes was tested in hamsters. Spermatozoa took up the added DNA by min and the uptake was inhibited by human seminal fluid in a dose dependent manner. Addition of DNA did not affect the functional competence of spermatozoa, in terms of their ability to undergo capacitation and acrosome reaction
US7169548B2 - Sperm cell processing and preservation systems - Google Patents
Egas Moniz, Lisbon, , Portugal. The impact of prion proteins in the rules that dictate biological reproduction is still poorly understood. Likewise, the role of prnt gene, encoding the prion-like protein testis specific Prt , in ram reproductive physiology remains largely unknown.
The analysis of freshly ejaculated semen is the most important diagnostic tool in the initial investigation of male fertility. Semen is a mixture of secretions from several components of the genital tract. During ejaculation, this fluid moves up through the epididymis and vas.